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  • Title
    Prevalence of Listeria species including L. monocytogenes from apparently healthy animals at Baroda Zoo, Gujarat State, India
    Journal Article
    Listeriosis is a infectious bacterial disease of domestic and wild animals and humans. A total of 56 faecal samples were collected from mammals and birds at Baroda Zoo, Vadodara, Gujarat State, India. Confirmation of the isolates was based on biochemical tests followed by phenotypic characterization by hemolysis on sheep blood agar, Christie Atkins Munch-Petersen (CAMP) test, phosphatidylinositol-specific phospholipase C (PI-PLC) assay and phosphatidylcholine-specific phospholipase C (PC-PLC) assay. The isolates were subjected to genotypic characterization with the help of polymerase chain reaction (PCR) assay for five virulence-associated genes, plcA, prfA, hlyA, actA and iap. Listeria monocytogenes isolates were further subjected to multiplex-PCR based serotyping. From 56 samples three (5.36%) were found positive for Listeria spp. of which one (1.79%) was identified as L. monocytogenes and two (3.57%) were identified as L. innocua. The isolate of L. monocytogenes was hemolytic, CAMP positive, PI-PLC positive, hlyA, pclA and prfA positive by PCR and turned out to be PC-PLC positive and was serotyped as 4b.
    Yadav M.M., Roy A., Bhanderi B., Jani R.G. (2011). Journal of Threatened Taxa 7(3) pp. 1929-1935; doi:10.11609/JoTT.o2094.1929-35